Project Fish-AI was invited to publish in the Methods in Molecular Biology, Springer Protocols’ special edition on Next Generation Culture Platforms for Reliable In Vitro Models.
In the framework of the book chapter UGent and UniMi partners stood up for a collaboration in order to help the scientific community to get a better insight in the keystones of the development of a life-like artificial in vitro intestine model.
The chapter discusses the importance of material design and development in order to achive the desired mechanical properties for the scaffolds in order to achive the most life-like version of the model possible from UGent partner’s view.
Example for detailed synthesis setup for gelatin-methacrylamide (Gel-MA) sythesis.
UniMi partner provided a short discussion on the relevance of primary cell line derivation from fish intestinal tissues and the combination of the cells with the materials designed by UGent in order to study the compatibility of the different components of the model.
What is more, the book chapter provides detailed protocols on the materials and methods used in the procedure extended with notes, which can help the kind readers to avoid the most common confusions with regards the preparation of the model.
The full text of the article is available at the publisher https://link.springer.com/protocol/10.1007%2F978-1-0716-1246-0_19
Nicole Verdile, Anna Szabó, Rolando Pasquariello, Tiziana A.L. Brevini, Sandra Van Vlierberghe, and Fulvio Gandolfi, Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols, Methods in Molecular Biology, vol. 2273, https://doi.org/10.1007/978-1-0716-1246-0_19